Portable Spectrophotometer, 12mm Caliber

Portable spectrophotometer ms3012 user manual

Q1: How does a colorimeter work?
A1: Colorimeters rely on the concept of the Beer-Lambert law, which assumes that the absorbance of a substance is proportional to its concentration. For example, the higher the concentration of a solution, the higher the absorbance reading. Many chemical and biological experiments are based on this concept. To obtain a Beer's law curve, several standards (solutions of known concentration) are prepared and their absorbance values are determined using a colorimeter.

Q2: What is colorimetry?
A2: A colorimeter is a device that measures light absorbance (how much light is absorbed) and light transmission (how much light passes through) in a liquid by analyzing the intensity of the color.

Q3: What are the limitations of a colorimeter?
A3: One limitation of chemical colorimeters is that differences in certain substances may lead to inaccurate test results. According to Global Water Instrumentation, because these differences are different for each substance, a chemical colorimeter alone is not a completely foolproof testing device.

Tips: Portable spectrophotometer working principle

Light Source: The instrument starts by emitting a beam of light. This light source can be a broad-spectrum white light LED or a specific wavelength source depending on the design.

Sample Interaction: The light from the source is then directed towards the sample placed within the measurement area. As the light interacts with the sample, different things can happen:

Absorption: Certain molecules within the sample may absorb specific wavelengths of light due to their electronic structure. This absorption removes that particular wavelength from the light beam.
Transmission: The remaining wavelengths that aren't absorbed will pass through the sample.

Wavelength Separation: The transmitted light (or reflected light in some models) is then directed through a diffraction grating or a prism. This component acts like a rainbow maker, separating the light into its individual wavelengths.

Detection: A detector array positioned behind the grating/prism measures the intensity of each separated wavelength. This essentially creates a digital fingerprint of how much light was absorbed or transmitted at each wavelength.

Data Analysis: The instrument's internal software analyzes the detector data. By comparing the intensity of the original light source with the intensity after interacting with the sample, the software can determine the absorption or reflectance spectrum of the sample.

The core principle of colorimeter revolves around analyzing the changes in the light caused by the sample. By measuring the missing or reduced intensity at specific wavelengths (absorption) or the remaining transmitted / reflected light intensity across the spectrum, the instrument reveals information about the sample's composition or properties.

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